FlowCam® - Flow Imaging Microscopy Blog

Analysis of Triacylglycerol (TAG) Algae Lipid Productivity with FlowCam®

High production rates of traicylglycerol (TAG), a lipid produced in microalgae, is a desirable trait in microalgae selection and cultivation.  TAG is used in a variety of applications ranging from biodiesel production to omega-3 fatty acid supplements.  

In a study published in Biotechnology for Biofuels (Cabanelas et al., 2016), Chlorococcum littorale, a strain of microalgae with increased triacylglycerol (TAG) productivity, was identified and analyzed. The cellular properties of the strain were imaged, favorable sub-populations were isolated, and confirmed for increased TAG productivity.The diameter, autofluorescence and lipid-dependent fluorescence of Chlorococcum littorale were measured and imaged using the FlowCam. Imaging flow cytometry confirmed that less-favorable strains with lower TAG productivity were removed using the developed strategy.

Access the article here.

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Cabanelas, I.T.D., van der Zwart, M., Kleinegris, D.M.M., Wijffels, R.H., Barbosa, M.J., 2016, Sorting cells of the microalga Chlorococcum littorale with increased triacylglycerol productivity. Biotechnology for Biofuels, 9:183.

Topics: Algae Technology, Aquatic Research