FlowCam^® - Flow Imaging Microscopy Blog

Back by popular demand, the FlowCam Student Equipment Grant program has a new category specifically for undergraduate institutions. The recipients of this inaugural undergraduate grant were Drs. Kerry Whittaker, LeAnn Whitney, and Steven Baer of the Corning School of Ocean Studies at Maine Maritime Academy (MMA), a small college of about 950 students located in Castine, ME.

Have you ever wondered how our technical customer support team performs quality control checks during services and before shipping new instruments? Here's one example of the process our team uses to check for accuracy on the FlowCam Cyano.

Pictured here: Enterprise Pond at YFT headquarters in Scarborough, Maine. The source of the sample used in this demonstration.

An interview with Steve Bowen, Director of Sales, Yokogawa Fluid Imaging Technologies

What is particle analysis?

Particle analysis involves taking a sample of a substance and analyzing the individual particles of the sample. The basic goal is to determine the constituents of a mixture and to differentiate between particles in the sample. Typical measurements of particles that are of interest include: particle size distribution, particle count, various measurements of particle shape, and particle concentration.

Determining whether algal cells are alive or dead is useful for a variety of applications including, but not limited to: wastewater analysis, algaecide testing, mesocosm experiments, and ballast water monitoring. Viability staining is a common approach used in flow cytometry to evaluate the relative abundance of live and dead cells in a sample. The FlowCam 8400, equipped with a laser, digital camera, and 2 channels of fluorescence detection, can be paired with various fluorescent stains to assess the viability of algal cells. Here we will describe how to pair an example of one such stain, fluorescein diacetate (FDA), with the FlowCam 8400 equipped with a 488nm blue laser.

After fielding many requests from FlowCam users, we are happy to announce that we have made available expanded taxonomic libraries. These example libraries illustrate some common genera found in Casco Bay (in the Gulf of Maine) and show examples of how users may organize libraries for their own unique area. The 42 libraries cover 37 different taxa.

Characterization of subvisible particles in protein therapeutics has become an increasingly important means to ensure the development of safe, stable, and effective medicines.  Many analytical techniques are available to identify and prevent not only protein aggregation, but to fulfill regulatory requirements. The purpose of this study by Danny Chou, President and Founder of Compassion BioSolution, LLC is to compare different techniques and their ability to identify subvisible particles and what relationship exists between the number of particles and different stress conditions imposed upon the protein drug products.

Flow Imaging Microscopy has been widely implemented for the analysis of particles ranging in size from 1-10µm. Yet, in recent years the FDA has highlighted the need for better analytical tools to fully understand how different stress conditions can impact the stability of the formulation. While light obscuration and membrane microscopy have been the primary methods to conduct USP testing for particles between 10µm - 25µm, regulators are now moving towards expecting orthogonal analytical methods to characterize and provide quantitative data on particles in the 2µm - 10µm range as well.

FlowCam Nano offers the ability to image and analyze particles ranging from 30µm down to as small as 300 nm.

The team at the University of Colorado, Boulder have long been FlowCam customers, most recently incorporating FlowCam images with artificial intelligence, in the form of convolutional neural networks, to characterize and identify impurities in protein therapeutics and cell based medicines.

Hunting Energy Services (HES), Subsea Technologies Division, is a manufacturer of subsea hydraulic products for the oil and gas industry. Their suite of hydraulic valve products includes applications for drilling control, production control, and Intervention Workover Control Systems (IWOCS).

Cell-based medicinal products (CBMPs) are gaining importance as therapeutic treatments for many life-threatening diseases. This study was designed to develop a method to characterize CBMPs using flow imaging microscopy (FIM) for the detection, quantification, and characterization of subvisible particulate impurities.  Recently, FIM has been applied to study cell viability and confluency in cell culture as well as quality of CBMPs. Comprehensive particle characterization can be limited when using the morphological parameters derived from the embedded software due to the complexity of CBMPs, which may consist of multiple particulate populations of highly heterogeneous morphologies.

Yokogawa Fluid Imaging Technologies' distributor to India's Biopharmaceutical market is Bioscreen Instruments. Bioscreen's Field Application Specialist, Jamuna Yadhav, shared with us the following testimonial and case study about their experience using the FlowCam to analyze injectable drug samples.