Flow Imaging Microscopy Blog

Microplastics in Our Oceans: How Can We Study These Microscopic Pollutants?

Microplastics are ubiquitous. They result from the breakdown of larger plastic waste (plastic bottles, bags, straws, glitter, fishing nets, toothbrushes, etc.) as well as synthetic fabrics (spandex, nylon, polyester, etc.). Research has discovered these micropollutants in our oceans, our shellfish, our bottled water. However, these tiny plastic particles can be challenging to study and therefore remain vastly uncharacterized.

microplastics imaged by FlowCamAbove: Microplastics imaged by the FlowCam. 

Methodologies for the detection, characterization, and quantification of microplastics continue to be developed. In response to the need to automate microplastic characterization, some researchers have turned to the FlowCam® imaging flow cytometer to image, characterize, and quantify them. The FlowCam was used in two recent studies to quantify, image, and measure microplastics.

Microplastics in Blue Mussels

Woods et al. (2017) from the Marine and Environmental Research Institute (Blue Hill, Maine) and Bigelow Laboratory for Ocean Sciences (Boothbay, Maine) quantified the uptake, digestion, and egestion of polyethylene terephthalate microplastic fibers (MPF) in blue mussels (Mytilus edulis) using the FlowCam. 

Microplastic Fibers FlowCam Woods et al 2017Above: Polyethylene terephthalate microplastic fibers (MPF) as imaged by the FlowCam. The crisp, non-weathered edges of the above MPF distinguished these from the naturally-occurring, weathered fibers from the lab environment during the study. Credit: Woods et al. (2017).

Woods et al. found that microalgal uptake rates were greatly reduced in mussels exposed to concentrations of 15,000 MPF/L or greater. Pseudofeces production showed positive correlation with MPF uptake rates at 30,000 MPF/L. A single fecal pellet showed up to 70 MPF.

Better Microplastic Research Using Digestion

Lorenz et al. (2017) from the Alfred Wegener Institute for Polar and Marine Research evaluated the efficacy of microplastic isolation in a natural marine water sample using a six-step enzymatic oxidative digestion developed by Löder et al. (2017). 

Microplastic_digestion_efficiency_Lorenz et al 2017

Lorenz et al. observed that the complete digestion process (all six steps) resulted in a 98.6% reduction in total particle area. Following the digestion, the remaining particulate matter was filtered from the water matrix. The total area of particulate matter per mL of sample was calculated using the FlowCam to determine an appropriate volume to be filtered so as not to overburden the filter. 

Above: The six-step digestion used by Lorenz et al. to isolate microplastics from organic matter in marine water samples. Each step shows a reduction in area of remaining particulate matter, as calculated by VisualSpreadsheet using FlowCam images. Credit: Lorenz et al. (2017). 

Read about the studies of Woods et al. and Lorenz et al. in detail. Download the poster presented at the 2018 Ocean Sciences Meeting in San Diego, California. 

Download the Poster "Use of Imaging Flow Cytometry (FlowCam) in the Study of  Microplastics"

Learn more about how the FlowCam is used for aquatic research around the globe, from microplastics characterization to phytoplankton population monitoring. 


References:

Woods, M.N., Stack, M., Fields, D.M., Matrai, P., (2017) Microplastic fber uptake, ingestion, and egestion rates in the blue mussel (Mytilus edulis). Unpublished raw data, Marine and Environmental Research Institute, Blue Hill, ME, and Bigelow Laboratory for Ocean Sciences, Boothbay, ME.

Lorenz, C., Speidel, L., Primpke, S., Gerdts, G., (2017) Using the FlowCam to Validate an Enzymatic Digestion Protocol Applied to Assess the Occurrence of Microplastics in the Southern North Sea. MICRO 2016: Fate and Impact of Microplastics in Marine Ecosystems doi: 10.1016/B978-0-12-812271-6.00094-6.

Löder, M.G.J., Imhof, H.K., Ladehoff, M., Löschel, L.A., Lorenz, C., Mintenig, S., et al. (2017) Enzymatic Purifcation of Microplastics in Environmental Samples. Environ Sci Technol 51:14283-14292 doi:10.1021/acs.est.7b03055. 

Topics: Marine Research, FlowCam Technology, Aquatic Research