FlowCam - Flow Imaging Microscopy Blog

Meet FlowCam Nano: Submicron Particle Imaging and Sizing

Nano Gen 2 transparent background

We’re excited to announce the next-generation FlowCam Nano! The newest member of our FlowCam instrument family detects and images particles between 300 nm and 2 µm—the smallest objects visible by light microscopy.

FlowCam Nano can be used to determine the types of submicron particles present in samples, such as biotherapeutic particles or aggregates, drug delivery vehicles, bacterial cells, and other nanoparticles of interest. If not addressed, many of these smaller particles are prone to agglomeration into larger subvisible and visible particles. FlowCam Nano may be used to identify and mitigate these smaller particles before larger ones will form. Other species like bacterial cells inherently exhibit a size near or below the lower size limit of FlowCam 8000 and will only be detected by FlowCam Nano.

Particle Size Range - FlowCam 8000 and Nano

Particulates in the submicron size range are not only missed by traditional flow imaging microscopy but are often challenging to characterize with other particle analysis instruments, many of which are either ensemble techniques or do not provide morphology information.

FlowCam Nano & 8100 images

Particles imaged for our white paper, "Early Detection of Aggregation in Formulations: FlowCam Nano for submicron particle characterization in biotherapeutics". Equivalent spherical diameter values for each particle are shown below their image.  

FlowCam Nano White Paper Thumbnail

In our new white paper, we demonstrate the analysis capabilities of FlowCam Nano for biotherapeutic formulation development. This study compares the results from FlowCam Nano
with those from FlowCam 8100 when analyzing samples containing protein aggregates, sucrose particles, and bacteria. We also demonstrate the consistency of the particle concentration measurements with FlowCam Nano and how the instrument may also be used to determine relative particle concentrations in samples.

This information can help users obtain a comprehensive picture of the particles in their sample, especially when paired with instruments like FlowCam 8100 and FlowCam LO.

Download White Paper

FlowCam Nano pushes FIM to the limits of light microscopy by employing a patented oil immersion-based flow imaging microscopy (FIM) approach to collect particle images at 40X magnification. Visit our FlowCam Nano products page to learn more or watch our video
on how flow imaging microscopy works.

Topics: Protein Therapeutics, News and Events, FlowCam Technology, Biopharmaceutical Research, Nanoparticles