FlowCam - Flow Imaging Microscopy Blog

The FlowCam was born from a dream to develop a faster flow cytometer for marine phytoplankton research. In the mid-1990s, the best method for analyzing phytoplankton was using optical microscopy or a slow flow cytometer that could only analyze a limited size range. By 1997, a few scientists from Bigelow Laboratory for Ocean Science had developed the first FlowCam prototype and debuted it at the ASLO Aquatic Sciences Meeting in Santa Fe, New Mexico. Today, 22 years later, the FlowCam has been used in over 230 aquatic science peer-reviewed publications. We've summarized our Top 4 recently published FlowCam for Marine Research Papers into one document.  The following papers are featured:

We welcome Stefanie Hebert as our new Accounting Assistant! 

Stefanie joins us with 8 years of experience in the finance department of a non-profit. There she mastered contract and budget development for the organization's many programs.

In her free time she enjoys painting, learning new DIY home improvements, and hanging out with her children. 

We're excited to have Stefanie on our team. Welcome, Stef! 

March 2019 — Harmful algal bloom (HAB) season is fast approaching. The City of Wichita Falls, Texas, however, has developed an integrated approach to monitor HABs that has prevented taste and odor events for the past two years.  Featured in the December 2018 issue of OpFlow, and now in the Jan/Feb 2019 issue of Texas H2O, the City of Wichita Falls shares its integrated approach to answer the following questions: 

Women represent half of the world's population however are still underrepresented in STEM fields. Less than 30% of researchers worldwide are women and only around 30% of all female students select STEM-related fields in higher education. As part of a world-wide effort to promote the full and equal participation of women in the sciences, the United Nations recognizes February 11 as International Women and Girls in Science Day. 

In December 2018, U.S. Congress submitted a letter signed by 61 Congressional Members to the Office of Management and Budget to lobby for increased funding to support harmful algal bloom (HAB) research in 2020.

HAB events are widespread and their effects are diverse. Coordinated studies released in 2017 by the Environmental Protection Agency, U.S. Geological Survey, and National Oceanic and Atmospheric Administration showed that 39% of all lakes nationwide contained toxic algae, and cyanobacteria-produced toxins were present in 78% of those lakes at some point during the year. Additionally, 40% more HAB events were reported in 2018 than in 2017. 

A study by Baker Hughes demonstrates that the FlowCam^® imaging particle analysis technology is a more informative method than spectrophotometry to evaluate the demulsification of produced water. Produced water generated during oil extraction is held in skim tanks where it is treated with water clarifiers or demulsifiers. Reverse emulsion breakers (REBs) coalesce the oil into larger molecules to be skimmed, or removed, from the produced water. The efficacy of REBs and other water clarifiers on produced water is important because oil extraction companies must meet water quality environmental regulations before releasing produced water back into the environment, or they require a low oil content if the water is to be reused in the extraction process. 

The 4th Annual European Workshop on Protein Aggregation and Immunogenicity will be held in Salzburg-Wals, Austria on January 28 and 29.  This conference serves as a European summit for thought leaders and academic researchers to come together and discuss the issues of protein aggregation and its effects on the immunogenicity of therapeutic protein drug products. We are excited to attend and exhibit the FlowCam imaging particle analyzer with our German distributor, Anasysta.

Proteins and contaminants from a parenteral drug sample, imaged and analyzed by the FlowCam.

We will soon be releasing VisualSpreadsheet (ViSP) 5.0, a significant advancement of the FlowCam software.  With ViSP 5.0 you will be able to organize your FlowCam files in a database format, allowing you to analyze multiple runs simultaneously as well as compare and contrast data sets.  This will be especially useful for time-series analyses, longitudinal studies, trend analysis, etc.

Proteinaceous particles in parenteral drugs pose an immunogenic risk. These formulations are therefore rigorously characterized for optimal conformational and colloidal stability of the drug molecule. As such, they undergo thorough analysis of biophysical descriptors and extended particle characterization to ensure a safe and stable product is delivered to market with a shelf life of about two years. In this post, we summarize a recent paper by Mattison et al. (2018) published in BioProcess International on how they successfully reformulated biotherapeutics by using quantitative stability predictors and descriptors. 

We use our Flow Imaging Microscopy blog as a platform to serve up the latest in relevant news, highlight novel uses of the FlowCam, and announce FlowCam technology developments. This year we blogged about 54 different topics. Below is a recap of the Top 10 Most Read Blog Posts of 2018.